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No. Publication Number Title Publication/Patent Number Publication/Patent Number Publication Date Publication Date
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1 US10527550B2
Method and microplate reader for investigating biological cells or cell cultures
Publication/Patent Number: US10527550B2 Publication Date: 2020-01-07 Application Number: 13/800,467 Filing Date: 2013-03-13 Inventor: Gebetsroither, Harald   Gfrorer, Andreas   Koota, Juha   Assignee: TECAN TRADING AG   IPC: G01N21/64 Abstract: A method for investigating biological cells or cell cultures in a microplate reader receives at least one microplate with wells containing biological cells or cell cultures by a receiving device; positions the wells with respect to measuring devices of the microplate reader and detects integral measurement signals by at least one of the measuring devices. The biological cells or cell cultures in the specific wells of the microplate(s) are transilluminated by an illumination source of the microplate reader and imaged by an imaging camera. Each of the detected integral signals is compared with the image of the biological cells or cell cultures in the corresponding wells of the microplate(s) by a processor and is related to the imaged number, adherence, confluence or morphology of these biological cells or cell cultures.
2 US10564100B1
Analysis of viable and nonviable cells
Publication/Patent Number: US10564100B1 Publication Date: 2020-02-18 Application Number: 16/159,482 Filing Date: 2018-10-12 Inventor: Moroz, Ekaterina   Apfel, Christian   Anderson, Kraig K.   Assignee: SageMedic Corporation   IPC: G01N21/64 Abstract: Provided are methods, for selectively analyzing a cell sample. Viable cells are dyed with a membrane-permeable fluorescent dye, and nonviable cells are dyed with a membrane-impermeable fluorescent quenching dye. The cells are illuminated to cause fluorescent emission from the membrane-permeable fluorescent dye in the viable cells and the membrane-impermeable fluorescent quenching dye in the non-viable cells. The cells are then quenched for at least a portion of fluorescence of the membrane-permeable fluorescent dye in the nonviable cells by the membrane-impermeable fluorescent quenching dye. The cells are then analyzed for viable and nonviable cells.
3 US10732107B2
Optical sensor, capnography system and methods of use
Publication/Patent Number: US10732107B2 Publication Date: 2020-08-04 Application Number: 15/502,186 Filing Date: 2015-08-07 Inventor: Victor, John C.   Morris, Michael J.   Shahriari, Mahmoud R.   Assignee: TELEFLEX MEDICAL INCORPORATED   IPC: G01N21/64 Abstract: The present application describes an optical sensor for measuring oxygen gas levels in a medium. The optical sensor includes a substrate having a first and second surface. The optical sensor also includes a first coating applied on the first surface of the substrate. The first coating may include an oxygen impermeable matrix doped with a first fluorophore. The optical sensor may include a second coating applied on the substrate. The present application also describes a capnography system for measuring oxygen including an optical sensor and an algorithm to estimate the maxima of oxygen levels from instantaneous oxygen levels and calculating instantaneous carbon dioxide levels from the difference between average maximum oxygen gas level and instantaneous oxygen gas level.
4 US2020116636A1
METHOD AND SYSTEM FOR ANALYSING FLUOROSPOT ASSAYS
Publication/Patent Number: US2020116636A1 Publication Date: 2020-04-16 Application Number: 16/627,029 Filing Date: 2018-06-27 Inventor: Smedman, Christian   Jalden, Joakim   Pelikan, Daniel   Del, Aguila Pla Pol   Magnusson, Klas   Assignee: MABTECH PRODUCTION AB   IPC: G01N21/64 Abstract: Disclosed is a method for analysing FluoroSpot assays. The method comprises illuminating a well of an assay plate with at least one excitation light, capturing at least one image of the well, in raw image format, for each excitation light, generating a model of analyte release distribution in the well for each excitation light, and clustering a plurality of co-positioned fluorospots as a multiple secretion fluorospot, wherein the clustering is performed for all generated models, and wherein the clustering determines at least one multiple secretion fluorospot. The generation of the model of analyte release distribution for a given excitation light comprises deconvolving the captured at least one image of the well to estimate a pre-diffusion analyte distribution, and detecting potential analyte release sites based on local maxima therein.
5 US2020116635A1
ANALYSIS OF VIABLE AND NONVIABLE CELLS
Publication/Patent Number: US2020116635A1 Publication Date: 2020-04-16 Application Number: 16/600,339 Filing Date: 2019-10-11 Inventor: Moroz, Ekaterina   Apfel, Christian   Anderson, Kraig K.   Assignee: SageMedic Corporation   IPC: G01N21/64 Abstract: Provided are methods, compositions, and kits for selectively analyzing a sample for viable cells and nonviable cells. For example, the method may include dyeing viable cells and/or nonviable with a membrane-permeable fluorescent dye, and dyeing nonviable cells with a membrane-impermeable fluorescent quenching dye. The method may include illuminating to cause fluorescent emission from the membrane-permeable fluorescent dye in the viable cells and the membrane-impermeable fluorescent quenching dye in the non-viable cells. The method may include quenching at least a portion of fluorescence of the membrane-permeable fluorescent dye in the nonviable cells by the membrane-impermeable fluorescent quenching dye. The method may include selectively analyzing the sample for the viable and nonviable cells.
6 US2020011796A1
OPTICAL MODULE FOR MULTI-WAVELENGTH FLUORESCENCE DETECTION
Publication/Patent Number: US2020011796A1 Publication Date: 2020-01-09 Application Number: 16/504,221 Filing Date: 2019-07-06 Inventor: Wang, Zhicai   Fu, Wenbo   Zhang, Yili   Assignee: Guangzhou Labsim Biotech Co., Ltd.   IPC: G01N21/64 Abstract: An optical module for multi-wavelength fluorescence detection includes an excitation light source component having at least one excitation light channel and including a first dichroic mirror and at least one light source for generating irradiation light; a color filter component for forming multiple fluorescence transmission paths; and a fluorescence receiving component having multiple fluorescence receiving channels. The first dichroic mirror is arranged at an irradiation light path of respective light sources. The color filter component is arranged at a transmission light path of the first dichroic mirror and includes at least one second dichroic mirror for receiving fluorescence signals. The fluorescence receiving component includes a plurality of optical elements, which are respectively arranged at a reflection and/or transmission light path of the second dichroic mirror. The application enables the simultaneous and instantaneous detection of multi-wavelength fluorescence.
7 CN111380848A
一种高光谱活体荧光分子成像系统及方法
Under Examination
Publication/Patent Number: CN111380848A Publication Date: 2020-07-07 Application Number: 202010297668.2 Filing Date: 2020-04-16 Inventor: 王懋   吴东岷   范娜娜   汪远   Assignee: 南京微纳科技研究院有限公司   IPC: G01N21/64 Abstract: 本发明的一种高光谱活体荧光分子成像系统包括用于承载样品的载物台和用于控制处理的计算机,载物台置于暗室箱体中,载物台上设有照明系统和生命维持系统,载物台的上方设有光源组件,载物台上方还设有用于接收样品受激发后发射荧光的接收装置,接收装置包括依次排列的滤光装置、成像镜头、面阵光电探测器和图像采集器,滤光装置为液晶可调谐滤波器或滤光片组,接收装置能够快速切换其透光波段以接收透光波段内受激发后发射的荧光,对样品进行荧光成像,同时通过快速采集每个波段激发荧光的光谱信息实现高光谱成像。该系统滤光波长连续可调,能提高光谱成像分辨率。本发明还提出一种高光谱活体荧光分子成像方法。
8 CN110376170B
基于CB7的养殖用水中恩诺沙星的快速测定方法
Valid
Publication/Patent Number: CN110376170B Publication Date: 2020-07-10 Application Number: 201910593507.5 Filing Date: 2019-07-03 Inventor: 钱鸣蓉   杨华   张校铭   汪建妹   吉小凤   Assignee: 浙江省农业科学院   IPC: G01N21/64 Abstract: 本发明公开了一种基于CB7的养殖用水中恩诺沙星的快速测定方法,包括步骤:取水样,加入恩诺沙星使其呈不同浓度,调节pH至3.0‑5.0,加入CB7,混合均匀,避光放置至少15min后得到待测溶液,用荧光分光光度计分别检测待测溶液以及同等条件下未加入CB7的待测溶液在激发波长278nm下发射波长445nm处发射峰的荧光强度,分别对应记做CB7增敏荧光强度、未增敏荧光强度,基于恩诺沙星与CB7作用后荧光光度的增强制作标准曲线,用于测定恩诺沙星含量。该方法对恩诺沙星具有很好的选择性,检测过程需要时间短,能够快速测定,操作简单,对检测人员的要求不高且能够实现对水中恩诺沙星的定量检测,利于大范围的推广应用。
9 CN108519361B
一种荧光光谱鉴别交趾黄檀和奥氏黄檀树种的方法
Valid
Publication/Patent Number: CN108519361B Publication Date: 2020-07-03 Application Number: 201810318463.0 Filing Date: 2018-04-11 Inventor: 王金砖   杜海慧   刘彬   梁峙   赵颖峰   赵越   张玉洁   吴海娟   Assignee: 南京市产品质量监督检验院   IPC: G01N21/64 Abstract: 本发明公开了一种交趾黄檀和奥氏黄檀树种鉴别的检测方法,包括如下步骤:1)交趾黄檀和奥氏黄檀中荧光物质提取条件的确立步2)根据1)确定的交趾黄檀和奥氏黄檀荧光物质的提取条件获得荧光物质,采用分子荧光光谱技术对提取的荧光物质进行荧光特征信息测定,构建交趾黄檀和奥氏黄檀树种荧光特征谱图库;3)将实际检测的荧光特征图谱与2)构建的交趾黄檀和奥氏黄檀树种荧光特征谱图库进行比对鉴别交趾黄檀和奥氏黄檀。本发明通过获取交趾黄檀和奥氏黄檀树种的荧光化合物并对其荧光特征信息进行测定,并结合传统的木材微观和宏观显微结构识别技术,对交趾黄檀和奥氏黄檀树种进行准确鉴定。该技术是一种更加科学、高效的木材树种鉴定新方法。
10 CN210923477U
一种水质检测装置
Valid
Publication/Patent Number: CN210923477U Publication Date: 2020-07-03 Application Number: 201921408870.7 Filing Date: 2019-08-28 Inventor: 冯发青   周绍均   Assignee: 冯发青   IPC: G01N21/64 Abstract: 本实用新型提供一种水质检测装置,包括光源驱动单元、水路系统、数据采集处理单元、结果显示单元、电源单元和荧光检测装置,光源驱动单元、水路系统和数据采集处理单元分别连接荧光检测装置,结果显示单元连接数据采集处理单元,电源单元给整个装置供电,荧光检测装置包括试样室、滤光片与光电二极管和紫外光源,滤光片与光电二极管和紫外光源分别安装在试样室外壁上,本实用新型设计合理,使用方便,检测过程中不需要使用试剂,不会造成二次污染,可手持操作、检测速度快、灵敏度高、耗电量低,有很好的应用前景。
11 CN111157505A
一种检测溶液中含硫污染物巯基乙酸的方法
Under Examination
Publication/Patent Number: CN111157505A Publication Date: 2020-05-15 Application Number: 202010050246.5 Filing Date: 2020-01-17 Inventor: 李妍   张俊华   张菲   孟洁   张子彤   杨妍   刘延舒   Assignee: 天津师范大学   IPC: G01N21/64 Abstract: 本发明公开了一种基于铜纳米簇荧光探针定量检测环境中含硫污染物巯基乙酸(TGA)的方法。本发明以谷胱甘肽为保护剂制备高性能的铜纳米簇荧光探针,基于三重态诱导荧光猝灭机制,通过目标物对该探针的荧光猝灭作用,以一种“turn‑off”的检测模式实现了对环境样品中污染物巯基乙酸含量的免标记、高灵敏、特异性检测。
12 CN111157499A
一种荧光检测仪器的校准方法
Under Examination
Publication/Patent Number: CN111157499A Publication Date: 2020-05-15 Application Number: 202010010067.9 Filing Date: 2020-01-06 Inventor: 丁卫东   苏四美   Assignee: 深圳市华科瑞科技有限公司   IPC: G01N21/64 Abstract: 本发明提供了一种荧光检测仪器的校准方法,该荧光检测仪器的校准方法采用现有的经过计量确定的标准设备进行定标操作,以该定标操作保证具有标准可追溯性和确定性,其能适合大批量和精确测量和提高荧光检测仪器在批量生产过程中的质量一致性,此外,该方法还可用于对荧光检测仪器进行质控以及保证荧光检测仪器性能参数的一致性。
13 CN111122529A
金纳米簇在检测生物样品中蛋白质淀粉样纤维化及筛选抑制剂的应用
Under Examination
Publication/Patent Number: CN111122529A Publication Date: 2020-05-08 Application Number: 201911389487.6 Filing Date: 2019-12-30 Inventor: 张耀东   郭英   马雪娟   夏梦凡   蒲莉   孙鹏跃   Assignee: 陕西师范大学   IPC: G01N21/64 Abstract: 本发明公开了金纳米簇在检测生物样品中蛋白质淀粉样纤维化及筛选抑制剂的应用。本发明以溶菌酶为模型蛋白,研究发现,金纳米簇的荧光强度随着溶菌酶纤维浓度的增大而增强,并且在一定的浓度范围内溶菌酶纤维浓度与荧光强度呈线性关系,因此可将金纳米簇应用于生物样品中蛋白质淀粉样纤维化过程的检测。与利用传统有机染料硫黄素T(ThT)的荧光法相比,金纳米簇具有低毒性、大Stocks位移的优点,且金纳米簇的荧光信号随蛋白质纤维化的程度变化灵敏,可以更灵敏地检测蛋白质由纤维化初期到对数生长期的转变,可更有效地研究蛋白质淀粉样纤维化过程和机理,并可用于筛选蛋白质淀粉样纤维化的抑制剂。
14 CN107884380B
一种微反应腔阵列式芯片的全自动快速荧光分析方法
Valid
Publication/Patent Number: CN107884380B Publication Date: 2020-05-12 Application Number: 201711369695.0 Filing Date: 2017-12-19 Inventor: 李桦楠   帕维尔·诺伊茨尔   徐颖   张浩卿   Assignee: 西北工业大学   IPC: G01N21/64 Abstract: 本发明属于微流控图像处理领域,特别是一种微反应腔阵列式芯片的全自动快速荧光分析方法。该方法利用图像区域划分——图像拼接——信号采集与分析的思想来提取并分析荧光信号,拼接图片时,先将第一行第二行图片拼接起来,然后将第三行图片拼接起来,再与第一二行所拼接成的图片拼接,再进行下一行的拼接,提高了后期荧光检测准确率;该方法实现了微反应腔阵列式芯片的全自动快速荧光分析过程中的全自动化,并且快速提取微反应腔阵列式芯片上的荧光信息,避免杂散光信号的干扰,不仅提高了检测结果的精确度和检测效率,而且为整个系统的快速化、高效化、集成化发展提供了思路。
15 CN111157507A
一种荧光光谱鉴别檀香紫檀木材树种的方法
Under Examination
Publication/Patent Number: CN111157507A Publication Date: 2020-05-15 Application Number: 202010147073.9 Filing Date: 2020-03-05 Inventor: 王金砖   杜海慧   梁峙   刘彬   赵颖峰   袁慧雯   赵金龙   陈瑶佳   Assignee: 南京市产品质量监督检验院   IPC: G01N21/64 Abstract: 本发明公开了一种檀香紫檀木材树种鉴别的检测方法,包括如下步骤:1)选择不同来源的檀香紫檀木材样品,对其存在的荧光化学物质以及选择合适的化学试剂对存在的荧光物质进行有效提取;2)采用分子荧光光谱技术对提取的化学物质进行荧光光谱分析,寻找最佳的测试条件,获取完整的檀香紫檀的荧光特征信息;3)采用确定的化合物提取方法和分子荧光光谱技术测定待测檀香紫檀木材树种荧光特征信息,确定具体的木材树种。本发明通过获取檀香紫檀树种的荧光化合物并对其荧光特征信息进行测定,并结合传统的木材微观和宏观显微结构识别技术,对檀香紫檀树种进行准确鉴定。该技术是一种更加科学、高效的木材树种鉴定新方法。
16 CN107817231B
一种针对大肠菌群以及大肠埃希菌的便携式检测仪
Publication/Patent Number: CN107817231B Publication Date: 2020-04-21 Application Number: 201710953765.0 Filing Date: 2017-10-13 Inventor: 王洪亮   王振华   范志浩   杨贤伟   马佳健   李盛勇   陈文浩   丁英杰   蔡佳玲   郑国娟   黄灿灿   潘健   沈国金   蔡强   Assignee: 浙江清华长三角研究院   济南海能仪器股份有限公司   IPC: G01N21/64 Abstract: 本发明公开了一种针对大肠菌群以及大肠埃希菌的便携式检测仪,可实现现场快速测定大肠菌群、大肠埃希菌,检测灵敏度可达到2×10mol,检测误差小于±5%,可以储存20000个检测结果,1000个采样计划,每个采样计划可以实现3000个采样点。相对传统表面皿培养法测定细菌数量需要24小时,本发明仅需10秒以内;与传统的台式设备相比本发明具有体积小,仅重380克,具有经济、方便、快捷、省时、敏感性高等优点,给现场监测带来了极大的便利,不但节省了大量人力、物力,而且还可扩大监测覆盖面,是基层卫生防疫机构对食饮具进行卫生监督的一种较理想的检测设备。
17 CN107271410B
细菌或真菌的活性快速检测方法
Publication/Patent Number: CN107271410B Publication Date: 2020-04-14 Application Number: 201710348799.7 Filing Date: 2017-05-17 Inventor: 吕斌   刘燕婕   李宁   叶磊   吴中乔   邓耘   项阳   Assignee: 武汉汉瑞隆德检测技术有限公司   IPC: G01N21/64 Abstract: 本发明涉及生物技术领域,尤其涉及一种细菌或真菌的活性快速检测方法。细菌或真菌的活性快速检测方法,包括步骤:1)首先配制多巴胺/丹磺酰多巴胺混合溶液;然后将多巴胺/丹磺酰多巴胺混合溶液、靶细胞和载体混合均匀,在载体表面进行聚合反应得到靶细胞‑丹磺酰多巴胺/多巴胺‑载体复合体;最后利用洗脱剂将靶细胞从复合体上洗脱下来,得到靶细胞的荧光分子印迹聚合物;2)向荧光分子印迹聚合物中加入样品后即刻测定荧光值为I0,待样品中的细菌或真菌与荧光分子印迹聚合物结合完全后,测定荧光值为I,I0/I‑1表示荧光变化水平,通过荧光变化水平确定样品中靶细菌或靶真菌的含量。该方法能快速准确地检测出样品中细菌或真菌的活性。
18 CN107907517B
基于荧光寿命的薄膜材料热物性测量系统及方法
Publication/Patent Number: CN107907517B Publication Date: 2020-04-10 Application Number: 201711310944.9 Filing Date: 2017-12-11 Inventor: 岳亚楠   吴昊   熊扬恒   Assignee: 武汉大学   IPC: G01N21/64 Abstract: 本发明公开了一种基于荧光寿命的薄膜材料热物性测量系统及方法,所述薄膜材料热物性测量系统包括探测光源、加热光源、光子探测器、时间相关单光子计数器和计算单元;探测光源用来照射薄膜样品以对薄膜样品进行荧光激发;加热光源用来照射薄膜样品以对薄膜样品进行瞬态加热;光子探测器用来探测荧光激发的荧光信号;时间相关单光子计数器与光子探测器信号连接,用来记录荧光激发的单光子信号;计算单元与时间相关单光子计数器信号连接,用来根据时间相关单光子计数器记录的单光子信号进行薄膜样品热物性计算。本发明简单易操作,测量方式灵活,灵敏度更高,可应用于生物、医学等领域,能在保证生物活性的同时,实现微小温度扰动下的热物性测量。
19 CN111007045A
一种耐火材料混合均匀度示踪剂
Under Examination
Publication/Patent Number: CN111007045A Publication Date: 2020-04-14 Application Number: 201911162458.6 Filing Date: 2019-11-25 Inventor: 刘步周   郁立超   余广田   Assignee: 无锡诚智炼钢辅助材料有限公司   IPC: G01N21/64 Abstract: 本发明公开了一种耐火材料混合均匀度示踪剂,包括以下成分:二硫化钼、发光萤石粉、光激发剂和三氧化二铬。本发明的耐火材料混合均匀度示踪剂,以三氧化二铬为主要原料,配入需要加入该组分的耐火材料中研究,可以避免污染耐火材料而造成浪费,所用氧化铬绿粒度为微米级,能够模拟耐火材料中主要使用的AlO微粉、SiO微粉等的混合特性;为了加强分散效果,加入了二硫化钼;而为了在对混合均匀度要求不高的情况下,用肉眼观察进行定性表征,本发明还加入了少量的荧石粉和光激发剂,简化了定量测试的过程,提升了测试速度,还降低了成本。
20 CN111007051A
基于激光诱导荧光光谱的蜂蜜检测方法及其检测装置
Under Examination
Publication/Patent Number: CN111007051A Publication Date: 2020-04-14 Application Number: 201911402004.1 Filing Date: 2019-12-30 Inventor: 陈和   张寅超   陈思颖   郭磐   徐启祥   Assignee: 北京理工大学   IPC: G01N21/64 Abstract: 本发明公开了一种基于激光诱导荧光光谱的蜂蜜检测方法及其检测装置,方法包括以下步骤:将待测蜂蜜与水充分混合,形成预定浓度的蜂蜜水溶液,激发波长为250nm到550nm的固定波长脉冲或连续激光器照射所述蜂蜜水溶液以诱导产生荧光,所述荧光通过滤光片进入到光谱仪以得到所述蜂蜜水溶液的荧光光谱,归一化所述荧光光谱,将其在蜂蜜荧光光谱数据库中比较以图形匹配其蜂蜜类型,如果存在匹配蜂蜜类型则确定待测蜂蜜为该类型,当存在至少两个匹配蜂蜜类型或不存在匹配蜂蜜类型时,掺假蜂蜜和样品组降维到低维度,基于明氏距离或kNN方法判断掺假蜂蜜的掺假浓度。